| dc.description.abstract | Background: As infection with Acquired Immune-deficiency Virus (HIV) befalls endemic in
Uganda; the risk of vulvovaginal candidiasis (VULVOVAGINAL CANDIDIASIS (VVC) )
as one of the most opportunistic infections remains high. Despite this, little is known
regarding the prevalence, candida speciation and their antifungal susceptibility profile. This
study sought to determine the prevalence of VULVOVAGINAL CANDIDIASIS (VVC) and
antifungal susceptibility pattern among HIV-infected women attending Touch Namuwongo
Project (TNP) at International Hospital Kampala.
Materials and methods: This was a hospital based cross section study involving 150 HIV-
positive women attending TNP during the months of May to July, 2017. Data was collected
using self-administered questionnaire and laboratory analyses of two high vaginal swabs
(HVS) for VULVOVAGINAL CANDIDIASIS (VVC) . Wet preparation, Gram stain, yeast
culture, antifungal susceptibility testing using the disk diffusion method, and identification
using phenotypic methods like germ tube test and Chrom ID agar were done. CD4 T-
lymphocyte for the previous three-months was obtained from hospital records. Data was
presented as proportions and the Chi-square test was used where a p-value of <0.05 was
considered.
Results: The prevalence of VULVOVAGINAL CANDIDIASIS (VVC) was 30.7% (95%
Confidence Interval 18.6-39.4). Majority of study participants had C.albicans (n=33, 71.1%).
Other Candida species isolated were; C. glabrata (8.7%), C. krusei (17.4%) and C.tropicalis
(2.2%). The antifungal susceptibility pattern of Candida species revealed that majority of the
strains isolated was either intermediate or resistant to the antifungal agents. Of the 46
participants with Candida species, we found a statistical association with a low immunity
shown by CD4 cell counts below 249cells/μL of blood (Odds ratio=4.8, 95% Confidence
Interval 3.7-5.1), declining clinical state as designated by WHO stages III and IV (Odds
ratio=2.9, 95% Confidence Interval 1.7-3.4) and not being on anti-retroviral therapy (ART)
(Odds ratio=4.2, 95% Confidence Interval 3.1-6.2).
Conclusion: We report a high prevalence of VULVOVAGINAL CANDIDIASIS (VVC) ,
with C. albicans as the most specie. Candida antifungal susceptibility testing is recommended
to avoid treatment failures. Also resistance to azole drugs such as fluconazole, the most
frequent antifungal used in the country could necessitate routine fungal culture and in-vitro
drug susceptibility testing. | en_US |
